Journal: Cancer biology & therapy

Article Title: The combination of 5-fluorouracil plus p53 pathway restoration is associated with depletion of p53-deficient or mutant p53-expressing putative colon cancer stem cells.

doi: 10.4161/cbt.8.22.10446

Figure Lengend Snippet: Figure 1. Upregulation of p53-dependent transcriptional activity or endogenous pro-apoptotic TRAIL receptor DR5 expression following p53 pathway restoration. In the upper panels, a whole population of Phoenix cells expressing a p53-responsive EGFP reporter show increased EGFP reporter expression following infection by a p73-expressing Adenovirus (upper right vs. upper left). In the lower panels, mutant p53-expressing human colon cancer DLD1 cells were treated with 15 μg/ml of the mutant p53 conformation modifying drug CP-31398 for 12 hours followed by fluorescence microscopy for p53 target TRAIL receptor DR5 expression by immunofluorescence with a DAPI nuclear counterstain. The lower left panel shows DR5 expression in untreated DLD1 cells and the lower right panel shows DR5 expression in CP-31398 treated DLD1 cells.

Article Snippet: A rabbit anti-human DR5 IgG antibody (Imgenex) was added at a 1:100 dilution and this was incubated overnight with gentle shaking at 4°C.

Techniques: Activity Assay, Expressing, Infection, Mutagenesis, Fluorescence, Microscopy, Immunofluorescence